摘要
      我们评估了脉冲电场（PEF）技术作为污水处理厂（STP）中一种新的消毒选择的适用性，该技术可以灭活抗生素抗性细菌（ARB）和抗生素抗性基因（ARGs）。结果表明，PEF消毒不仅能灭活万古霉素耐药肠球菌（VRE），还能灭活vanA耐药基因。应用PEF消毒可以有效地灭活可培养的VRE，并将其降低到检测限以下（VRE的log降低值>5 log）。尽管vanA也显示出超过4log的减少，但仍保持在105拷贝/mL的数量级，这表明在PEF应用的消毒中，ARG比ARB更难被灭活。在本研究中验证的每个施加条件下的参数中，发现初始电压对ARB和ARGs的失活最重要。此外，频率是影响持续时间增加或减少的参数，建议通过增加频率来缩短治疗时间。我们的研究结果强烈表明，PEF应用消毒可能是STP的一种新的消毒技术选择，有助于控制水生环境中的ARB和ARGs污染。
Abstract
We evaluated the suitability of pulsed electric field (PEF) technology as a new disinfection option in the sewage treatment plants (STPs) that can inactivate antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). It was shown that PEF applied disinfection could inactivate not only vancomycin-resistant enterococci (VRE), but also vanA resistance gene. Cultivable VRE could be effectively inactivated by PEF applied disinfection, and were reduced to below the detection limit (log reduction value of VRE > 5 log). Although the vanA also showed a reduction of more than 4 log, it remained in the order of 105 copies/mL, suggesting that ARGs are more difficult to be inactivated than ARB in PEF applied disinfection. Among parameters in each applying condition verified in this study, the initial voltage was found to be the most important for inactivation of ARB and ARGs. Furthermore, frequency was a parameter that affects the increase or decrease of the duration time, and it was suggested that the treatment time could be shortened by increasing the frequency. Our results strongly suggested that PEF applied disinfection may be a new disinfection technology option for STPs that contributes to the control of ARB and ARGs contamination in the aquatic environments.

https://www.sciencedirect.com/science/article/pii/S0304389421023505