摘要
      文献报道了污泥的热水解过程（THP）对厌氧消化（AD）过程中抗生素抗性基因（ARGs）去除的积极影响。然而，关于THP引起的不同胞外聚合物（EPS）的变化如何影响AD期间ARG的繁殖，目前几乎没有可用的信息。本研究的重点是系统地关联THP预处理的污泥（80°C、110°C、140°C、170°C）的EPS组分和AD中ARG的丰度。在生化甲烷电位（BMP）测试中，不同条件下的THP改善了污泥的溶解，随后提高了甲烷产量。最高甲烷产量为275 ± 对于THP-140°C，观察到11.5 ml CH4/g COD，即40.5 ± 比对照高2.5%。由于回弹效应，THP操作温度的升高显示出AD中ARG传播的非线性响应。在140°C下，THP对AD中ARGs的去除率最高。多元分析表明，除大环内酯类耐药基因外，EPS多糖与大多数ARG和整合素呈正相关。相反，EPS蛋白仅与β-内酰胺抗性基因强相关。这些结果表明，针对特定EPS成分操纵THP操作条件对于有效缓解AD中特定ARG类型的传播至关重要。
Abstract
The positive impact of the thermal hydrolysis process (THP) of sewage sludge on antibiotic resistance genes (ARGs) removal during anaerobic digestion (AD) has been reported in the literature. However, little information is available on how changes in different extracellular polymeric substances (EPS) due to THP can influence ARG propagation during AD. This study focused on systematically correlating EPS components and ARG abundance in AD of sewage sludge pretreated with THP (80 °C, 110 °C, 140 °C, 170 °C). THP under different conditions improved sludge solubilization followed by improved methane yields in the biochemical methane potential (BMP) test. The highest methane yield of 275 ± 11.5 ml CH4/g COD was observed for THP-140 °C, which was 40.5 ± 2.5% higher than the control. Increasing THP operating temperatures showed a non-linear response of ARG propagation in AD due to the rebound effect. The highest ARGs removal in AD was achieved with THP at 140 °C. The multivariate analysis showed that EPS polysaccharides positively correlated with most ARGs and integrons, except for macrolides resistance genes. In contrast, EPS protein was only strongly correlated with β-lactam resistance genes. These results suggest that manipulating THP operating conditions targeting specific EPS components will be critical to effectively mitigating the dissemination of particular ARG types in AD.

https://www.nature.com/articles/s41598-022-10764-1