摘要
      使用八个全尺寸厌氧消化器，探讨了去除抗生素抗性基因（ARGs）和intI1的效率。消化器根据基质类型（食物垃圾、粪肥或污泥）表现出不同的特性；配置（单级或两级）；温度（嗜冷性、中温性或嗜热性）；水力停留时间（HRT）（9.7–44天）；和操作模式（连续搅拌釜反应器或塞流反应器）。与输入底物的类型相比，消化器的配置或操作参数对ARGs丰度的影响更大。冗余分析（RDA）占总方差的85.2%，具有相同配置和操作条件的蒸煮器在去除ARG方面表现出相似的性能。在HRT相对较长（32天）的两级高温蒸煮器中观察到去除ARG的最高效率（99.99%）。在具有相对较短HRT（9.7天）的单级中温条件下观察到最低的去除效率（97.93%），这可能是由于垂直和水平基因转移。
Abstract
Efficiencies of removing antibiotic resistance genes (ARGs) and intI1 were explored using eight full-scale anaerobic digesters. The digesters demonstrated different characteristics on the basis of substrate types (food waste, manure or sludge); configuration (single or two-stage); temperature (psychrophilic, mesophilic or thermophilic); hydraulic retention time (HRT) (9.7–44 days); and operation mode (continuous stirred tank reactor or plug flow reactor). Digesters’ configuration or operating parameters showed a greater effect on abundance of ARGs than the type of input substrate. Redundancy analysis (RDA) accounted for 85.2% of the total variances and digesters with the same configuration and operational conditions showed similar performance for removal of ARGs. The highest efficiencies of removing ARGs (99.99%) were observed in two-stage thermophilic digesters with relatively long HRTs (32 days). The lowest removal efficiency (97.93%) was observed in single-stage mesophilic with relatively short HRTs (9.7 days), likely due to vertical and horizontal gene transfer.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422002589