摘要
      研究了在梯度增加（0.5至10mg/L）和减少暴露（10至0.5mg/L）模式下，微生物群落和抗生素抗性基因（ARGs）对阿奇霉素和铜联合污染的反应。在梯度增加暴露模式下，硝化作用受到更明显的抑制。在两种暴露模式下，古菌群落和功能结构的反应都比细菌更明显。优势细菌和古菌组成（Hyphomicrobium、Euryarchaeota等）受到两种暴露模式的影响，但一些罕见的古菌（Methanoregula和Methanosarcina）除外。细菌和古菌之间存在更多的正相关性，硝化螺旋菌是关键属。氨氧化古菌（0.37–3.06%）和完全氨氧化菌（Nitrospira_ENR4）富集，硝化菌与反硝化基因（napA/B、nosZ等）密切相关。在两种暴露模式下，检测到50种ARG亚型，特异性ARG亚类型（aac、ImrA等）增殖。细菌和古菌是24种ARGs的常见宿主，并对它们的转移起到了作用。
Abstract
The responses of microbial communities and antibiotic resistance genes (ARGs) to azithromycin and copper combined pollution under gradient increasing (from 0.5 to 10 mg/L) and decreasing exposure (from 10 to 0.5 mg/L) modes were investigated. Nitrification was inhibited more obviously under gradient increasing exposure mode. Responses of archaeal community and function structure were more obvious than bacteria under both exposure modes. The dominant bacterial and archaeal compositions (Hyphomicrobium, Euryarchaeota, etc.) were affected by two exposure modes, except some rare archaea (Methanoregula and Methanosarcina). There were more positive correlations between bacteria and archaea, and Nitrospira was keystone genus. Ammonia-oxidizing archaea (0.37–3.06%) and complete ammonia oxidizers (Nitrospira_ENR4) were enriched, and Nitrososphaera_viennensis was closely related to denitrifying genes (napA/B, nosZ, etc.). 50 ARG subtypes were detected and specific ARG subtypes (aac, ImrA, etc.) proliferated in two exposure modes. Bacteria and archaea were common hosts for 24 ARGs and contributed to their shifts.

https://www.sciencedirect.com/science/article/abs/pii/S0960852421019106