摘要

由于其简单性和成本效益，湿地建设是一种推荐的家庭污水处理技术。冬季和夏季调查了10年内稳定运行的综合表面流人工湿地（ICW）中14种抗生素抗性基因（ARG）的浓度。观察到生活污水是ICW中ARG的主要来源，ICW中总目标ARG的去除率分别在冬季和夏季分别达到77.8％和59.5％。在整个治疗过程中，夏季的5种ARGs（sul1，tetA，tetC，tetE和qnrS）以及6种ARGs（sul1，sul3，tetA，tetC，tetE和qnrS）的浓度增加。 ARGs在水中和沉积物中发现的相关性强，特别是在夏季，表明ARG可能在水和沉积物之间交换。在intI1和几个ARGs浓度之间也观察到强烈的正相关，这意味着流动性遗传因子可能在ICW中传播ARGs中起关键作用。我们的研究结果表明水性ARG可以通过ICW被有效去除，并且ICW也可以充当特定ARG的储库。

Wetland construction is a recommended domestic sewage treatment technique, owing to its simplicity and cost efficiency. Concentrations of 14 antibiotic resistance genes (ARGs) in an integrated surface flow constructed wetland (ICW) steadily operated over 10 years were investigated in the winter and summer. Domestic sewage was observed to be the primary source of ARGs in the ICW, and 77.8% and 59.5% removal rates of total targeted ARGs in the ICW were achieved in the winter and summer, respectively. Concentrations of five ARGs (sul1, tetA, tetC, tetE, and qnrS) in the winter and of six ARGs (sul1, sul3,tetA, tetC, tetE, and qnrS) in the summer were increased throughout the treatment process. Strong correlations were found between ARGs in water and those found in sediments, especially in the summer, indicating that ARGs may be exchanged between water and sediment. Strong positive correlations were also observed between concentrations of intI1 and several ARGs, implying that mobile genetic elements may play a key role in the dissemination of ARGs in an ICW. Our study results suggest aqueous ARGs could be effectively removed via an ICW and that ICWs can also act as reservoirs of specific ARGs.

https://www.sciencedirect.com/science/article/pii/S0045653517300309