摘要

在与庆大霉素和洛伐他汀发酵残余物（分别为GFR和LFR）的共堆肥过程中评估了细菌群组成中的动态，以及13种抗生素抗性基因（ARG）和8种移动遗传因子（MGE）。 使用新一代测序技术，确定了与堆肥不同阶段相关的关键细菌类群。 最重要的是，属于厚壁菌门的芽孢杆菌属与增强的庆大霉素降解，有机物质分解（OM）和溶解有机碳（DOC）以及堆肥循环的嗜热阶段的延长有关。 在堆肥过程中，不同的ARG / MGE的模式各不相同。 然而，总数和标准化（对细菌数量）拷贝都很高。 各种ARG的丰度与细菌丰度和群落组成有关，单个ARG的变化模式受MGEs和细菌选择性的影响。

Dynamics in bacterial community composition, along with 13 antibiotic resistance genes (ARGs) and eight mobile genetic elements (MGEs), were assessed during co-composting with gentamicin and lovastatin fermentation residue (GFR and LFR, respectively). Using next generation sequencing, the key bacterial taxa associated with the different stages of composting were identified. Most importantly, Bacillus, belonging to Phylum Firmicutes, was associated with enhanced degradation of gentamicin, decomposition of organic matter (OM) and dissolved organic carbon (DOC), and also extension of the thermophilic phase of the composting cycle. During the course of composting, the patterns of different ARGs/MGEs varied. However, the total and the normalized (to bacterial numbers) copies both remained high. The abundance of various ARGs was related to bacterial abundance and community composition, and the changing pattern of individual ARGs was influenced by the selectivity of MGEs and bacteria.

https://www.sciencedirect.com/science/article/pii/S096085241830511X