摘要
目标
鲍曼不动杆菌是一种医院内病原体，表现出多药耐药性，是全世界关注的主要问题。因此，我们旨在评估临床菌株鲍曼不动杆菌KBN10P05679的基因组特征，以阐明其抗生素耐药性机制和毒力因素。
方法
进行了计算机多点序列分型、系统发育鉴定、基因组注释、基因组分析、抗生素敏感性测试和生物膜形成分析，并研究了抗生素耐药性和生物膜相关基因的表达水平。
后果
KBN10P5679的全基因组包括一个3990428bp的环形染色体和两个质粒（74294和8731bp），并被分配到ST451序列类型。同源基因簇注释鉴定了3810个基因，包括参与氨基酸运输和代谢、转录、无机离子运输、能量生产和转化、复制、重组和修复以及碳水化合物和蛋白质代谢的基因。通过检索综合抗生素耐药性数据库对抗生素耐药性基因进行了研究，发现基因组中含有30个不同的抗生素耐药性基因。对毒力因子数据库的分析揭示了KBN1005679基因组中的86个毒力因子基因。KBN10P5679菌株被发现具有更高的生物膜形成能力，并且比其他测试菌株以更高的水平表达生物膜相关基因。
结论
本研究中获得的抗生素耐药性基因型和潜在毒力因子相关数据将有助于指导未来的研究，以制定这种耐多药病原体的控制措施。
ABSTRACT
Objectives
Acinetobacter baumannii, a nosocomial pathogen, exhibits multidrug resistance and is a major concern worldwide. We therefore aimed to evaluate the genomic features of the clinical strain A. baumannii KBN10P05679 to elucidate its antibiotic resistance mechanisms and virulence factors.

Methods
In silico multilocus sequence typing, phylogenetic identification, genome annotation, genome analysis, antibiotic susceptibility testing, and biofilm formation assay were performed, and the expression levels of antibiotic resistance- and biofilm-related genes were investigated.

Results
The complete genome of KBN10P05679 comprises a circular chromosome of 3 990 428 bp and two plasmids (74 294 and 8731 bp) and was assigned to the ST451 sequence type. Clusters of Orthologous Gene annotation identified 3810 genes, including those involved in amino acid transport and metabolism, transcription, inorganic ion transport, energy production and conversion, replication, recombination and repair, and carbohydrate and protein metabolism. The antibiotic resistance genes were investigated by searching the Comprehensive Antibiotic Resistance Database, and the genome was found to harbour 30 different antibiotic resistance genes. Analysis of the Virulence Factor Database revealed 86 virulence factor genes in the KBN1005679 genome. The KBN10P05679 strain was found to have a higher capacity for biofilm formation and expressed biofilm-related genes at a higher level than the other tested strains.

Conclusions
The antibiotic resistance genotype and potential virulence factor-related data obtained in this study would help direct future studies for developing the control measures for this multidrug-resistant pathogen.

https://www.sciencedirect.com/science/article/pii/S2213716523000322